Abstract
Objective: The aim of the present study was to investigate the protective efficacy of rotenone against lipopolysaccharide (LPS) and D-galactosamine (D-GalN)-induced altered hematology.
 Methods: Hematotoxicity was induced by coinjection of LPS (50 μg/kg i.p.) and D-GalN (300 mg/kg i.p.). Rotenone (5, 10 and 20 mg/kg p.o.) was administered for 6 days as a pre-treatment. Blood was collected through puncturing the retro-orbital sinus to analyze blood parameter. Serum was separated to analyze glucose, triglyceride, and cholesterol.
 Results: The present study revealed decreased in red blood cells, platelets, hemoglobin, and hematocrit value while a significant increase in white blood cells, lymphocyte, and monocytes were observed in LPS and D-GalN treated rats. LPS and D-GalN administration significantly decrease glucose level while serum lipid profile (triglycerides and cholesterol level) were increased significantly at 5% level of significance. LPS and D-GalN-induced altered hematological and serological variables were restored toward control by rotenone pretreatment for 6 days in dose-dependent manner.
 Conclusion: It can be said that LPS and D-GalN administration resulted in alteration of various hematological parameters and rotenone at 20 mg/kg dose restored significant alteration toward control due to the presence of antioxidant activity of rotenone.
Highlights
Increased oxidative stress alters the balance between pro-oxidants and antioxidants, which is been linked to many human diseases or disorders at present [1]
Antioxidative defense system (AOS) components are responsible for neutralizing this oxidative stress and protect various cell damages [2]
More than 80% recovery was observed in almost all the parameters with 20 mg/kg dose of rotenone
Summary
Increased oxidative stress alters the balance between pro-oxidants (free radicals) and antioxidants, which is been linked to many human diseases or disorders at present [1]. Antioxidative defense system (AOS) components are responsible for neutralizing this oxidative stress and protect various cell damages [2]. Afolabi 2011 [11] suggested that stress induced by environmental, nutritional, and pathological factors could alter hematological parameters. Lipopolysaccharide (LPS) is one of the main components of the bacterial cell wall and is responsible for generating oxidative stress [12] and inflammation [13]. D-GalN is reported to mediate inflammationinduced toxic damage in animals [17]. Rotenone could be effective against LPS and D-GalN-induced hematotoxicity
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
