Protective effect of rosiglitazone reperfusion injury in rats

Abstract

Objective To study the protective effect of different doses of rosiglitazone （RSG） against focal cerebral ischemia-reperfusion injury in rats. Methods Forty male Sprague Dawley rats were randomly divided into the following groups： sham-operation, control, low- dose RSG （1 mg/kg ·d）, moderate-dose （2 mg/kg · d）, high-dose （4 mg/kg· d） groups （n = 8 in each group）. A rat model of middle cerebral artery occlusion for 2 hours and reperfusion for 22 hours were induced by the intraluminal suture method. An automatic biochemical analyzer was used to detect the blood glucose and lipid levels. A solid phase sandwich enzyme-linked immunosorbent assay was used to determine serum insulin levels, and the insulin sensitivity indexes were calculated. The effect of rosiglitazone on infarct vohmae and behavioristics was observed. Results Compared with the control group, the infarct volume was significantly reduced, and the neurological function scores were improved significantly in each RSG gcoup （P 〈 0. 05 or P 〈 0.01）. The insulin sensitivity was sigtificantly increased （P 〈0. 05 or P 〈0. 01）, and hypergly- cemia was reduced significantly after cerebral ischemia （P 〈0.05） and showed a dose-dependent manner. Conclusions RSG has obvious neuroprotective effect against cerebral ischemia/ reperfusion injury in rats. Its mechanism may be associated with the increase of insulin sensitivity. Key words: Rosiglitazone; Thiazolidinediones; Brain ischemia; Reperfusion injury; Insulin resistance; Hyperglycemia; Neuroprotective agents; Rats