Abstract
BackgroundHyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Resveratrol and quercetin are red wine polyphenols with known beneficial cardiovascular properties, including an antioxidant capacity. This study evaluated whether resveratrol and/or quercetin could prevent in vitro-induced diabetic changes in neurogenic and vascular relaxant responses of mouse arteries and corpora cavernosa.MethodsIsometric tension of isolated aorta, mesenteric arteries and corpora cavernosa was measured using organ bath systems. Diabetic conditions were mimicked in vitro by co-incubating the tissues for 2 h with high glucose (HG, 30 mM) and MGO (120 µM).ResultsThe presence of HG and MGO significantly blunted acetylcholine (Ach)-induced relaxations in corpora cavernosa and mesenteric arteries but not in aorta. Electrical field stimulated (EFS) responses of corpora cavernosa were also significantly inhibited by these diabetic conditions. In corpora cavernosa 2 h co-incubation with resveratrol (30 µM) or quercetin (30 µM) significantly attenuated HG and MGO-induced deficits in Ach- and EFS-responses.ConclusionsOur study demonstrates that in mouse arteries, HG and MGO rather affect endothelium derived hyperpolarizing factor-mediated than nitric oxide (NO)-mediated relaxations. In corpora cavernosa HG and MGO interfere with NO release. Resveratrol and quercetin protect mouse corpora cavernosa from diabetic-induced damage to NO-mediated relaxant responses. This might rely on their antioxidant capacity.
Highlights
Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes
To explore the role of endothelium derived hyperpolarizing factor (EDHF), Ach-curves were constructed in presence of the nitric oxide (NO) synthase inhibitor, Nω-nitro-l-arginine methyl ester hydrochloride (L-NAME) (0.1 mM, 20 min), and the cyclooxygenase blocker, indo (10 μM, 20 min)
This study demonstrates that high glucose (HG) in combination with MGO impairs endothelium- and neurogenic-mediated relaxations in mouse corpora cavernosa and mesenteric arteries which is prevented by antioxidants
Summary
Hyperglycemia and increased levels of methylglyoxal (MGO) can trigger the development of vascular complications in diabetes. Hyperglycemia and increased levels of methylglyoxal (MGO), a reactive metabolite produced during glycolysis, are two hallmarks of diabetes mellitus which can trigger the development of vascular complications in diabetes [1,2,3,4,5]. These vascular events are presumed to underlie the pathogenesis of diabetic-related erectile dysfunction (ED). The aim of this study was to evaluate whether resveratrol and/or quercetin could protect mouse mesenteric artery and/or corpora cavernosa from HG and MGO-induced defects in endothelium- and neurogenic-mediated relaxations
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