Abstract
PurposeThe purpose of this study was to determine the protective effects of Resveratrol (RESV) on acute bright light-induced retinal degeneration in aged senescence accelerated mouse strain.MethodsTen three-month-old male SAMP8 mice (prone to aging) were randomly assigned to two experimental dietary groups: one untreated group and one RESV treatment group (n=20 eyes for each group). After 30 days of treatment, mice were exposed to intense bright light. Ten male SAMR1 mice (resistant to aging) served as control (n=20 eyes). The protective effects of RESV administration on light-induced retinal degeneration in SAMP8 strain as well as the effect of bright light damage in the retinas of SAMP8 mice were analyzed by electroretinography (ERG), retinal histology, mRNA, protein and lipid profile.Results68%-85% of a-wave amplitude and 72%-92% of b-wave amplitude were persevered by RESV in SAMP8 mice that were exposed to light damage. Also, RESV preserved their photoreceptor nuclei. mRNA expression of neuroprotective factors leukemia inhibitory factor (LIF), brain derived neurotrophic factor (BDNF), oncostatin M (OSM), cardiotrophin 1(CT-1) and cardiotrophin-like cytokine (CLC) were up-regulated 28, 8, 7, 5 and 9-fold in SAMP8 mice after RESV treatment. In addition, RESV could suppress the NF-κB pathway by down-regulating the expression of pIκB. Light damage led to increase of saturated FA, monoenoic FA, n6 PUFA and n6/n3 ratio and decrease of Docosahexaenoic acid (DHA). There was no significant difference on DHA and the ratio of n6/n3-FA between the untreated and RESV treated SAMP8 mice.ConclusionsCollectively, our study provides evidence that RESV prevents light-induced retinal damage associated with aging.
Highlights
Aging is associated with an increasing prevalence of neurodegenerative disorders and leads to irreversible alterations in humans and experimental animals [1]
68%-85% of a-wave amplitude and 72%-92% of b-wave amplitude were persevered by RESV in SAMP8 mice that were exposed to light damage
Collectively, our study provides evidence that RESV prevents lightinduced retinal damage associated with aging
Summary
Aging is associated with an increasing prevalence of neurodegenerative disorders and leads to irreversible alterations in humans and experimental animals [1]. Among SAMP mice, SAMP8 mice have been extensively used in previous studies to investigate the effect of aging in different organs as mentioned above in pancreas These aging mice are considered as a good model of Alzheimer’s disease to study the fundamental mechanisms of age-associated deficiencies in learning and memory [9]. A number of striking similarities between Alzheimer’s disease and AMD have been described Both represent a complex and multifactorial degeneration of central nervous system in which aging is a primary risk factor [10]. Aged SAMP8 mice have been recently shown to develop retinopathy similar to some features of human AMD including Aβ deposition and increased inflammatory response in the outer retina [12]
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