Abstract
The present study was conducted to clarify the protective effect of Brazilian propolis ethanol extract (BPEE) against stress-induced gastric mucosal lesions in rats. The protective effect of BPEE against gastric mucosal lesions in male Wistar rats exposed to water-immersion restraint stress (WIRS) for 6 h was compared between its repeated preadministration (50 mg/kg/day, 7 days) and its single preadministration (50 mg/kg). The repeated BPEE preadministration attenuated WIRS-induced gastric mucosal lesions and gastric mucosal oxidative stress more largely than the single BPEE preadministration. In addition, the repeated BPEE preadministration attenuated neutrophil infiltration in the gastric mucosa of rats exposed to WIRS. The protective effect of the repeated preadministration of BPEE against WIRS-induced gastric mucosal lesions was similar to that of a single preadministration of vitamin E (250 mg/kg) in terms of the extent and manner of protection. From these findings, it is concluded that BPEE preadministered in a repeated manner protects against gastric mucosal lesions in rats exposed to WIRS more effectively than BPEE preadministered in a single manner possibly through its antioxidant and anti-inflammatory actions.
Highlights
Propolis is a resinous hive product collected by honeybee from various plant sources and it can be used for a wide range of purposes as anti-inflammatory, antioxidant, antibacterial, and immunomodulatory agents [1,2,3]
Our previous report has shown that a single preadministration of Brazilian propolis ethanol extract (BPEE) (50 mg/kg, p.o.) to Wistar rats protects against gastric mucosal lesions induced by 6 h of water-immersion restraint stress (WIRS) more effectively than a single preadministration of the extract (10 mg/kg or 100 mg/kg, p.o.) [20]
This finding has suggested that when excessive BPEE is preadministered to rats with WIRS, like the case of a single preadministration of BPEE (100 mg/kg), accumulation of unknown component(s) enhancing stress sensitivity in the preadministered BPEE in the body leads to an enhancement of stress sensitivity
Summary
Propolis (bee glue) is a resinous hive product collected by honeybee from various plant sources and it can be used for a wide range of purposes as anti-inflammatory, antioxidant, antibacterial, and immunomodulatory agents [1,2,3]. Propolis obtained from different areas of the world is constituted by 50–60% of resin, 30–40% of wax, 5– 10% of essential oils, and 5% of pollen, besides microelements [4] It contains various organic compounds such as phenols, tannins, polysaccharides, terpenes, aromatic acids, and aldehydes [1, 4, 5]. It has been reported that the hydroalcoholic extract of Brazilian green propolis, the main phenolic acids in the extract, and Baccharis dracunculifolia, the main botanical source of Brazilian green propolis, protect against gastric mucosal lesions induced by ethanol, indomethacin, or water-immersion restraint stress (WIRS) in rats [6,7,8] These reports have suggested that the hydroalcoholic extract of Brazilian green propolis could exert an antiulcer effect in rats with WIRS by reducing the volume and acidity of gastric juice and by increasing the pH of gastric juice [6,7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
