Protective effect of remote ischemic preconditioning by brief hind limb ischemia against the gastric mucosal injury caused by severe gastric ischemia‐reperfusion: participation of glucocorticoids (840.12)

Abstract

In the present study we investigated the effect of noninvasive hind limb ischemic preconditioning on the gastric mucosal injury caused by severe gastric ischemia‐reperfusion and participation of glucocorticoids in this effect. Under deep anesthesia rats were exposed to prolonged gastric ischemia‐reperfusion (30 min occlusion of celiac artery followed by 3 h of reperfusion) alone or with preliminary remote ischemic preconditioning (10 min hind limb ischemia, by placing a tourniquet on the proximal part of the limb, followed by 30 min of reperfusion). The participation of glucocorticoids was investigated by glucocorticoid receptor antagonist RU‐38486, inhibitor of glucocorticoids’ synthesis metyrapone, and adrenalectomy alone or with corticosterone replacement. The prolonged gastric ischemia‐reperfusion resulted in the gastric injury. In control animals limb remote ischemic preconditioning induced plasma corticosterone rise and attenuated the gastric injury caused by prolonged gastric ischemia‐reperfusion. In adrenalectomized rats without corticosterone replacement and in rats pretreated with metyrapone or RU38486 the gastroprotective effect of limb remote ischemic preconditioning was prevented. The results suggest that hind limb remote ischemic preconditioning attenuates gastric injury caused by severe ischemia‐reperfusion and glucocorticoids participate in the protective effect.Grant Funding Source: Supported by grants from RFBR (N13‐04‐01680‐a); Programs of Presidium RAS 7P