Protective Effect of Quercetin Nanoemulsion on 5-Fluorouracil-Induced Oral Mucositis in Mice.

Mandana Lotfi,Sohrab Kazemi,Fatemeh Shirafkan,Marzieh Pirzadeh,Mohammad Hosseini,Ali Akbar Moghadamnia,Anahita Ebrahimpour,Prasanna Kumar Santhekadur

doi:10.1155/2021/5598230

Mandana Lotfi, Sohrab Kazemi + Show 6 more

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https://doi.org/10.1155/2021/5598230

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Abstract

The target of this study was to evaluate the efficacy, histopathological, oxidative stress, and molecular effects of quercetin (QRC) in mice with oral mucositis induced by 5-fluorouracil (5-FU). Thirty-six albino male mice with oral mucositis induced by 5-FU as a chemotherapeutic agent were used in this study. The animals were randomly divided into 6 groups: control group, mucositis (MUC) group, pretreatment group, posttreatment group, and two last groups including nanoemulsion form of QRC with a dose of 5 mg/kg in both pretreatment and posttreatment. In the present evaluation, fewer oral lesions were observed in the QRC posttreatment groups compared to the pretreatment and nanoemulsion receiving groups. In the SOD assay, the most significant difference was observed in the posttreatment nanogroup (41.073 ± 1.24) and pretreatment nanogroup (43.453 ± 2.60) in comparison to the 5-FU group (30.897 ± 1.93). The results of CAT assay also showed a significant difference in nano-posttreatment (124.60 ± 10.85), posttreatment (135.4 ± 9.82), and nano-pretreatment groups (128.80 ± 7.20) compared to the 5-FU group (55.07 ± 8.91). The expression of inflammatory genes such as Hif-1α and NfκB in this group was lower than in the other groups, although this difference was not significant. It seems that the use of QRC can improve the treatment process of oral mucositis induced by 5-FU.

Highlights

Mandana Lotfi,1 Sohrab Kazemi,2 Anahita Ebrahimpour,2 Fatemeh Shirafkan,2 Marzieh Pirzadeh,1 Mohammad Hosseini,3 and Ali Akbar Moghadamnia 2
E target of this study was to evaluate the efficacy, histopathological, oxidative stress, and molecular effects of quercetin (QRC) in mice with oral mucositis induced by 5-fluorouracil (5-FU). irty-six albino male mice with oral mucositis induced by 5-FU as a chemotherapeutic agent were used in this study. e animals were randomly divided into 6 groups: control group, mucositis (MUC) group, pretreatment group, posttreatment group, and two last groups including nanoemulsion form of QRC with a dose of 5 mg/kg in both pretreatment and posttreatment
These symptoms can even interrupt the course of treatment [13, 14]. ese lesions start when the oral mucosa is exposed to chemical agents, causing DNA destruction and cell death. ey are commonly caused by the production of reactive oxygen species (ROS) and oxidative stress [15]

Summary

Introduction

E expression of inflammatory genes such as Hif-1α and NfκB in this group was lower than in the other groups, this difference was not significant It seems that the use of QRC can improve the treatment process of oral mucositis induced by 5-FU. 5-FU inhibits DNA synthesis during the S phase of the cell cycle by limiting the availability of thymidylate [2] It is used alone or in combination with other common drugs in the treatment of various cancers, including breast, head and neck, anal, stomach, colorectal, and some skin cancers [3,4,5]. 5-FU, like other anticancer drugs, has many side effects that reduce the patient’s quality of life by causing various destructive effects and sometimes interrupt treatment [6,7,8]. On the other hand, using NSAIDs to alleviate adverse reactions of chemotherapeutic agents is problematic and cannot be a good solution to treat and improve mucositis caused by cancer chemotherapy [25]

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