Protective effect of quercetin against in vitro erythrocyte rheology alterations produced by arsenic.

Abstract

Humans are exposed to heavy metals such as arsenic (As), through contaminated food and drinking water. The effect of As on RBC membrane is one of the most important biological effects. In a previous work, we have studied the AsVin vitro effect on erythrocytes biophysical properties discovering alterations regarding aggregability deformability, cell morphology, membrane fluidity and osmotic response. We have also observed that the presence of the metal produces an oxidative stress in RBCs that might be the origin of rheological impairment. In the present work we analyzed RBCs rheological properties associated with membrane fluidity and lipid peroxidation in presence of As and quercetin (Qc). From our results we can conclude that RBCs treatment with Qc is efficient to prevent the impairment of the mechanical properties of the cell membrane produced by the As, through oxygen reactive agents in the membrane structure, mainly on the lipids. This protective effect is observed in the preservation of the erythrocytes rheological properties and consequently in the maintenance of an appropriate blood flow, specially in the small vessels in the peripheral circulation.