Protective effect of pyrrolidine dithiocarbamate on the rat kidney from ischemia reperfusion injury

Abstract

Objective To investigate the protective mechanism of pyrrolidine dithiocarbamate (PDTC) on the kidney in the isehemia-reperfusion injury model (IRI).Methods Fifty-six health adult Wistar rats were randomly divided into three groups: IRI group (n = 24), PDTC group (n = 24, 150 mg/kg PDTC was administered before IRI), and control group (n = 8).Rats in IRI and PDTC groups were sacrificed at the 2nd, 6th and 24th h after IRI.Serum levels of urea nitrogen (BUN) and creatinine (Cr) were determined.Levels of IL-8, TNF-α, NF-κB and iNOS mRNA was detected in renal tissues.Histological changes were observed.Results At the 2nd, 6th and 24th h after IRI, levels of BUN, Cr, IL-8, TNF-α, NF-κB and iNOS mRNA in IRI group were increased significantly as compared with control and PDTC groups (P 0.05).No significant differences were found in all parameters between PDTC and control groups at the 24th h after IRI (P>0.05).In comparison to Irl group, renal pathological changes in PDTC group were alleviated significantly.Conclusion These findings indicate that PDTC pretreatment obviously attenuates IRI by down-regulating IL-8, TNF-α and iNOS through inhibiting NF-κB. Key words: pyrrolidines; kidney; Reperfusion injury; NF-κB