Abstract

 
 
 
 Purpose: To investigate the hepatoprotective effect of Ipomoea batatas extract against alcohol-induced liver damage in mice.
 Methods: Male C57BL/6 mice were randomly divided into 4 experimental groups (n = 10). Normal Group: The animals received distilled water 5 ml/kg for 7 days; Alcohol Group: The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Purple sweet potato leaf extract (PSPE) Group: PSPE 400 mg/kg was for 7 days. The animals received alcohol 5 ml/kg of 40 % w/v alcohol for 7 days; Alcohol + Hovenia dulcis Thunb extract (HDE) Group: HDE 400 mg/kg was for 7 days. To confirmed to the liver protection effect of PSPE, it was calculated, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), and total cholesterol (TC) in serum were detected. To evaluate changes of histological in alcohol-fed mice, liver tissue was determined by H&E staining.
 Results: Blood alcohol concentration in purple sweet potato leaf extract (PSPE) 200 mg/kg and Hovenia dulcis (H. dulcis) extract (HDE) 200 mg/kg treated group significantly decreased compared to - alcohol with water treated group (p < 0.05). Serum ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced. Liver sections in mice stained with H&E (hematoxylin and eosin) stain to displayed the physiological changes in the liver tissue. Furthermore, the results showed that inflammatory cells increased in the alcohol group compared to the normal group, but spontaneously decreased in the PSPE or HDE-treated group.
 Conclusion: These results demonstrate that Ipomoea batatas may be therapeutically effective in protecting the liver from alcohol-induced hepatotoxicity and fatty liver.
 
 
 
Highlights
Alcoholic liver disease (ALD) is a leading cause of liver disease and associated with significant disease and mortality worldwide, including Korea [1]
Despite the insightful economic and influence impact of ALD, the underlying mechanisms of Alcohol is mainly metabolized in the liver through three enzymatic paths: alcohol dehydrogenase (ADH), acetaldehyde dehydrogenase (ALDH) and cytochrome p450 2E1 (CYP2E1)
Since the liver serves as a major site for ethanol oxidation, there is ample evidence that acetaldehyde acts as a major cause of liver damage after chronic alcohol consumption [4]
Summary
Alcoholic liver disease (ALD) is a leading cause of liver disease and associated with significant disease and mortality worldwide, including Korea [1]. The present study was performed to investigate the possible effect of purple sweet potato leaf on alcohol induced-liver damage in mice. The experimental group were divided as follows: Normal Group: The animals received DW 5 ml/kg for 7 days; Alcohol Group: The animals received alcohol 5 ml/kg of 40% w/v alcohol for 7 days; Alcohol + Purple sweet potato leaf extract (PSPE) Group: PSPE 400 mg/kg was for 7 days; Alcohol + Hovenia dulcis Thunb extract (HD) Group: HDE 400 mg/kg. For pretreatment of the sample, 50 ml of methanol was added to 0.5 g of PSPE and extracted with a reflux extractor at 70°C for 1 h, and the supernatant obtained through centrifugation was placed in a 200 ml quantitative flask and filled up with distilled water (DW). Statistical significance (p < 0.05 for all analyses) was assessed by ANOVA with Instat 3.05 (GraphPad, San Diego, CA), followed by Student–Newman–Keuls analysis
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