PROTECTIVE EFFECT OF PARACALYX SCARIOSA (ROXB.) ALI AGAINST ETHANOL INDUCED HEPATIC TOXICITY IN BRL 3A CELL LINES

Abstract

Paracalyx scariosa (Roxb.) Ali. is a woody twiners belongs to the family Fabaceae. The use of plant has been mentioned in folk medicine to treat liver disorders. The review has been reported the presence of flavonoids like quercetin, rutin and kaempferol etc. There are scientific proofs available regarding the hepatoprotective effect of these flavonoids. However, no scientific record is available for the hepatoprotective activity of Paracalyx scariosa (Roxb.) Ali. Hence the present study was designed to evaluate the protective activity of Paracalyx scariosa (Roxb.) Ali against ethanol induced toxicity. Protective effect of extracts (methanol, benzene and acetone) and fractions (ethyl acetate, aqueous) of methanol was tested against ethanol induced toxicity in BRL 3A cell lines. The degree of hepatoprotection was determined by measuring cell viability, cytotoxicity marker LDH and hepatic marker enzymes AST, ALT and ALP. Silymarin was used as the standard drug for comparison. The extracts and fractions significantly reduced the ethanol induced cytotoxicity and elevated the reduced levels of LDH, AST, ALT, and ALP after treatment. The results were analyzed by one way ANOVA followed by Dunnett’s test. The present study suggests that the extracts (methanol, benzene and acetone) and fractions (ethyl acetate, aqueous) of Paracalyx scariosa (Roxb.) Ali. possess significant hepatoprotective activity which was found to be dose dependent in BRL 3A cell lines.