Protective Effect of Mussel (Mytilus Coruscus) Extract Containing Taurine Against AAPH-Induced Oxidative Stress in Zebrafish Model.

Abstract

The objective of this present study was to examine in vitro and in vivo antioxidative activities of mussel (Mytilus coruscus) water extract. Taurine concentration of mussel water extract was 140.76 nmol/mg, which was more abundant than all of the free amino acids combined. The antioxidative activity of the mussel extract was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. The half maximal inhibitory concentration (IC50) value of the mussel extract for DPPH and alkyl radical scavenging activity was 1.24 mg/mL and 0.44 mg/mL, respectively. Furthermore, we evaluated the protective effect of mussel extract against 2,2′-azobis dihydrochloride (AAPH)-induced oxidative stress in the zebrafish model. Mussel water extract containing taurine significantly reduced ROS generation, lipid peroxidation and cell death in the zebrafish model. These findings indicate that mussel water extract contains in vitro and in vivo antioxidant activities based on the zebrafish model.