Protective effect of Moringa oleifera and green tea aqueous extract in combination against beta-cyfluthrin induced mutagenicity in albino rats

Abstract

The present work was designed to investigate the mutagenicity effect of beta-cyfluthrin in bone marrow of male albino rats and the protective role of the combined aqueous extract of dried leaves of Moringa oleifera (MO) and green tea (GT). Twenty adult male rats were divided randomly into 4 groups, each containing five rats per group. The route of administration was orally gavage for 28 consecutive days. Rats in Group (a) were given distilled water and served as control, rats in Group (b) were given beta-cyfluthrin at a dose of 6.75 mg/kg b.wt (1/20 of estimated LD50). Rats in Group (c) were given aqueous extract of (MO+GT) as the only source of drinking fluid, for 28 days. Rats in Group (d) were given the same doses of group (b) and enabled continuously to an aqueous of (MO+GT) extract as the only source of drinking fluid. Animals were sacrificed humanely at the end of study. The micronucleus and chromosomal aberrations assays were used as mutagenicity tests. The results showed that beta-cyfluthrin administration alone increased the frequency of micronucleated polychromatic erythrocytes (MNPCEs) and induced several of chromosomal aberrations (CAs) in bone marrow cells of treated rats compared to control group. While the aqueous extract of (Mo+GT) in combination against the mutagenic potential of beta-cyfluthrin significantly decrease the (MNPCEs) and (CAs). In conclusion, this study confirmed the genotoxic potential of beta-cyfluthrin but also revealed the strong protective effect of aqueous leaves extract of Moringa oleifera and Green tea in combination as anti-mutagenic agents.