Protective effect of mitochondrial fission inhibitor on organ function in septic rat

Abstract

Objective To investigate the protective effect of mitochondrial fission inhibitor-1 (Mdivi-1) on organ functions in septic rats. Methods with cecal ligation and puncture (CLP) imitated sepsis model, 120 SD rats were divided into 5 groups randomly: sham, sepsis, conventional treatment, Mdivi-1 (1 mg/kg) and Mdivi-1 (3 mg/kg), the effects of different concentrations of Mdivi-1 on vital organ function of liver, kidney, intestines, cardiac, and animal survival were observed. Results As compared with the sham, the organ function was damaged after sepsis. After conventional treatment with LR, anti-infection and vasoactive drugs, organ functions were no significant improvement. The different concentration of Mdivi-1 (1 mg/kg and 3 mg/kg) in combination with conventional treatment significantly improved organ functions, 3 mg/kg Mdivi-1 had best effect. As compared with conventional treatment, aspartate aminotransferase (AST) (152.2±11.8 vs. 216.5±14.7, P=0.000), alanine aminotransferase (ALT) (49.0±3.8 vs. 66.3±2.3, P=0.000), blood urea nitrogen (BUN) (11.2±1.6 vs. 16.1±2.8, P=0.001), creatinine (Cr) (23.6±2.5 vs. 35.7±2.7, P=0.000), D-lac (1.3±0.1 vs. 2.8±0.3, P=0.000) the animal survival time was (60.30±30.82) h vs. (11.28±20.77) h (P=0.000), the survival rate was 8/16 vs. 1/16. Conclusion Mitochondrial fission inhibitor Mdivi-1 was beneficial to sepsis via improving multiple organ functions. Key words: Mitochondrial fission inhibitor-1; Sepsis; Rat; Organ function