Abstract

Ulcerative colitis (UC) is an inflammation-related disease involved in uncontrolled inflammation and oxidative stress and is characterized by high recurrence and relapse risk. As a rising star in gas medicine, methane owns the properties of anti-inflammation, antioxidation, and antiapoptosis. Based on the possible mechanism, we aimed to investigate the effect of methane on UC. Methane-rich saline (MRS) was introduced here, and UC was induced by acetic acid. All the C57BL/6 mice were allocated into groups as follows: control group, colitis model group, colitis treated with salazosulfapyridine (SASP) group, and colitis treated with MRS (1 or 10 ml/kg) groups. Tissue damage, the degree of inflammation, oxidative stress, and apoptosis were evaluated in the study, as well as the TLR4/NF-κB/MAPK and IL-10/JAK1/STAT3 signaling pathways for further exploration of the potential mechanism. The results showed that MRS (1) alleviated tissue damage caused by acetic acid, (2) controlled acetic acid-induced inflammation, (3) inhibited acetic acid-caused oxidative stress, (4) reduced colonic cell apoptosis due to acetic acid, (5) suppressed the TLR-4/NF-κB/MAPK signaling pathway, and (6) activated IL-10/JAK1/STAT3 anti-inflammatory response to improve the injury induced by acetic acid. We conclude that MRS has a protective effect on acetic acid-induced ulcerative colitis in mice via blocking the TLR4/NF-κB/MAPK signaling pathway and promoting the IL-10/JAK1/STAT3-mediated anti-inflammatory response.

Highlights

  • Ulcerative colitis (UC), which belongs to the chronic nonspecific inflammatory bowel disease (IBD) family related to immunization, is inflammation in the mucosa and subserosa of the colon and rectum

  • We found that the spleen weight of mice with colitis showed an increase compared with that of the control group, while SASP and methane-rich saline (MRS) significantly reduced the spleen weight of mice (Figure 1(a))

  • We found that the disease activity index (DAI) score of group AA was significantly higher than that of any other group, showing that SASP or MRS treatment could relieve the symptoms of UC

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Summary

Introduction

Ulcerative colitis (UC), which belongs to the chronic nonspecific inflammatory bowel disease (IBD) family related to immunization, is inflammation in the mucosa and subserosa of the colon and rectum. The main manifestations of UC are diarrhea, mucopurulent bloody stools, abdominal pain, recurrence, and relapse. Severe UC will result in complications such as a toxic megacolon and an increased risk of colorectal cancer. It was indicated that the prevalence and incidence of UC have been increasing gradually in recent years [1, 2]. UC has been calling for increasing concern because of its damage to individual lives and work capacity. The etiology of UC remains unclear, and multiple factors are considered, among which environmental factor, genetic factor, and microbial factor have gained wide acknowledgment

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