Abstract
The harm of nonalcoholic fatty liver disease to human health is increasing, which calls for urgent prevention and treatment of the disease. Isoorientin is an effective ingredient of Chinese herbal medicine with anti-inflammatory and antioxidant effects. However, the effect of isoorientin in nonalcoholic fatty liver disease is still unclear. In this study, combined in vivo and in vitro experiments, through pathological observation, flow cytometry, immunofluorescence and western blot analysis to explore the role of isoorientin in steatosis and reveal its molecular mechanism. The results demonstrated that oleic acid treatment significantly increased the content of ROS and lipid droplets in rat hepatocytes, and promoted the expression of γH2AX, HO-1, PPARγ, SREBP-1c, FAS. The ROS content in the cells of co-treated with isoorientin and oleic acid was significantly reduced compared to the oleic acid group, and the expression of γH2AX, HO-1, PPARγ, SREBP-1c, FAS, and the nuclear translocation of NF-κB p65 were also significantly inhibited. Our data showed that oleic acid induce oxidative damage and steatosis in hepatocytes both in vitro and in vivo, and activate the PPARγ/NF-κB p65 signal pathway. Moreover, isoorientin can significantly reduce oleic acid -induced oxidative damage and steatosis by regulating the PPARγ/NF-kB p65 signal pathway.
Highlights
Fatty liver disease refers to excessive accumulation of fat in liver cells, which is a serious threat to people’s health
The morphology of hepatocytes was improved, and the number of lipid droplets and vacuolation were decreased in the Oleic acid (OA) and ISO co-treatment group compared to the OA alone treatment group
We first detected the oxidative damage of OA to cells, and the results showed that the expression of γH2AX and HO-1 protein, and the concentration of intracellular reactive oxygen species (ROS) increased with increase of OA concentration and there were significant differences in the concentration of 1.2 mM OA compared to the control group (Figures 2A,B)
Summary
Fatty liver disease refers to excessive accumulation of fat in liver cells, which is a serious threat to people’s health. Oleic acid (OA) is the most abundant monounsaturated free fatty acid in the serum of nonalcoholic fatty liver. It exists in animals and plants, and is an indispensable nutrient in Isoorientin and Oleic Acid animal food. It plays a very important role in the occurrence and development of fatty liver (Jiao et al, 2020; Li L. et al, 2020). OA is an indispensable nutrient in animal food, excessive amounts can cause cell damage. Its specific molecular mechanism has not yet been elucidated
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