Protective Effect of Ischemic Postconditioning Against Testicular Ischemia-Reperfusion Injury in Rabbits

Abstract

PurposeTo investigate the protective effect of ischemic postconditioning (IP) against testicular ischemia/reperfusion (I/R) injury of different degrees in rabbits.Material & MethodsA total of 42 rabbits were randomly divided into seven groups: control group, different degree I/R group (R1, R2, R3),and different degree IP group (P1, P2, P3), six in each group. nder ultrasound monitoring, made of different degree of testicular ischemia model. Group R1 and group P1 showed homogeneous echo and flow signals slightly decreased, group R2 and P2 showed heterogeneous echo and flow obviously decreased, group R3 and P3 showed radial or fragmental low echo areas and flow disappeared. Before the long time reperfusion, group I/R had direct perfusion, group IP was subjected to perfusion for 30 s and then ischemia for 30 s, repeated three times. After 3 days of breeding, malonaldehyde (MDA) and superoxide dismutase (SOD) levels, and histology were measured.ResultsThe levels of MDA and SOD had significant differences between group R1 and P1 (p < 0.05), and between group R2 and P2 (p < 0.05), but there were no significant differences between group R3 and P3 (p > 0.05). Johnsen's score and apoptosis index had significant differences in group R1 and P1 (p < 0.05), but there were no significant differences in group R2 and P2 (p > 0.05), and in group R3 and P3 (p > 0.05).ConclusionIP can attenuate testicular damage induced by I/R, but it is restricted by the degree of ischemia. Scrotal ultrasound can be used to predict the effect of IP against testicular IR injury. PurposeTo investigate the protective effect of ischemic postconditioning (IP) against testicular ischemia/reperfusion (I/R) injury of different degrees in rabbits. To investigate the protective effect of ischemic postconditioning (IP) against testicular ischemia/reperfusion (I/R) injury of different degrees in rabbits. Material & MethodsA total of 42 rabbits were randomly divided into seven groups: control group, different degree I/R group (R1, R2, R3),and different degree IP group (P1, P2, P3), six in each group. nder ultrasound monitoring, made of different degree of testicular ischemia model. Group R1 and group P1 showed homogeneous echo and flow signals slightly decreased, group R2 and P2 showed heterogeneous echo and flow obviously decreased, group R3 and P3 showed radial or fragmental low echo areas and flow disappeared. Before the long time reperfusion, group I/R had direct perfusion, group IP was subjected to perfusion for 30 s and then ischemia for 30 s, repeated three times. After 3 days of breeding, malonaldehyde (MDA) and superoxide dismutase (SOD) levels, and histology were measured. A total of 42 rabbits were randomly divided into seven groups: control group, different degree I/R group (R1, R2, R3),and different degree IP group (P1, P2, P3), six in each group. nder ultrasound monitoring, made of different degree of testicular ischemia model. Group R1 and group P1 showed homogeneous echo and flow signals slightly decreased, group R2 and P2 showed heterogeneous echo and flow obviously decreased, group R3 and P3 showed radial or fragmental low echo areas and flow disappeared. Before the long time reperfusion, group I/R had direct perfusion, group IP was subjected to perfusion for 30 s and then ischemia for 30 s, repeated three times. After 3 days of breeding, malonaldehyde (MDA) and superoxide dismutase (SOD) levels, and histology were measured. ResultsThe levels of MDA and SOD had significant differences between group R1 and P1 (p < 0.05), and between group R2 and P2 (p < 0.05), but there were no significant differences between group R3 and P3 (p > 0.05). Johnsen's score and apoptosis index had significant differences in group R1 and P1 (p < 0.05), but there were no significant differences in group R2 and P2 (p > 0.05), and in group R3 and P3 (p > 0.05). The levels of MDA and SOD had significant differences between group R1 and P1 (p < 0.05), and between group R2 and P2 (p < 0.05), but there were no significant differences between group R3 and P3 (p > 0.05). Johnsen's score and apoptosis index had significant differences in group R1 and P1 (p < 0.05), but there were no significant differences in group R2 and P2 (p > 0.05), and in group R3 and P3 (p > 0.05). ConclusionIP can attenuate testicular damage induced by I/R, but it is restricted by the degree of ischemia. Scrotal ultrasound can be used to predict the effect of IP against testicular IR injury. IP can attenuate testicular damage induced by I/R, but it is restricted by the degree of ischemia. Scrotal ultrasound can be used to predict the effect of IP against testicular IR injury.