Abstract
Lungs retrieved from non‐heart‐beating donors (NHBD) might alleviate the shortage of suitable organs for transplantation. Since hypercapnia has protective effect on free radical damage, in present study we investigated a possible protective effect of hypercapnic ventilation during reperfusion in lungs retrieved from NHBD.Experimental rats underwent the protocol of NHBD lung harvesting: 90 minutes of warm ischemia after pentobarbital euthanasia followed by 60 min of cold ischemia (12°C). Then we measured perfusion pressure, lung weight gain and arterio ‐ venous difference in O2 partial pressure in isolated, Salt solution + Ficol perfused lungs. In controls, lungs were harvested immediately after euthanasia.We used four groups of rats: Control group – C Control group, isolated lungs ventilated with hypercapnic gas (10%CO2) ‐ CH Experimental rats – warm + cold ischemia – W Experimental rats, hypercapnic ventilation – WH Warm ischemia deteriorates lung functions (W vs C). We found that hypercapnic ventilation reduced pulmonary edema (WH vs. W) and improved oxygen transport ability (WH vs. W, no difference in WH vs. C). We conclude that hypercapnia prevents ischemia – reperfusion injury in NHBD lungs.Supported by Czech Science Foundation, GACR P303/10/P343 and GACR 305/08/0108
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