Protective effect of Gallic acid on doxorubicin-induced testicular and epididymal toxicity.

Abstract

The effect of Gallic acid (GA) on doxorubicin (DOX)-induced testicular and epididymal toxicity was investigated in experimental rat model. The rats were randomly divided into six groups of 10 animals per group. Rats in group A received clean tap water ad libitum. Rats in group B were administered DOX intraperitoneally at 15mg/kg on the eighth day of the experiment. Animals in groups C and D received 60 and 120mg/kg GA orally for 7days with 15mg/kg DOX on the eighth day. Rats in groups E and F received 60 and 120mg/kg GA alone orally for 7days. The animals were sacrificed 24hr after the last administration. DOX administration led to a significant (p<0.05) increase in hydrogen peroxide and malondialdehyde levels with significant reduction in antioxidant enzymes and reduced glutathione levels. DOX administration also led to a significant increase in total sperm abnormalities and prolactin together with a significant decrease in testosterone levels. Immunohistochemistry revealed higher expressions of caspase 3 in the testicular tissues of rats that received DOX alone. Together, pre-treatment with GA attenuated markers of oxidative stress, reversed sperm abnormality and ameliorated the observed aberration in plasma testosterone and prolactin levels.