Protective Effect of foliar application of sulfur on photosynthesis and antioxidative defense system of rice under the stress of Cd

Abstract

This paper investigates the effect of foliar application of sulfur on photosynthesis and antioxidative defense system of rice under the stress of Cd. The initial field studies showed that foliar spray of S was effective for reducing Cd concentration in rice and increasing the grain yield. However, the physiological mechanisms remain less clear on how the foliar application of S alleviates Cd toxicity in rice. Chlorophyll fluorescence, as a measure of photosynthesis, was taken to estimate the efficiency of photosystem II (PSII) photochemistry after the foliar application of S. The increase of photosynthetic parameters, i.e. the maximum photochemical efficiency of PSII reaction center (Fv/Fm), the actual PSII photochemical efficiency (ΦPSII), the photochemical quenching coefficient (qP), indicated that the foliar treatment alleviated the toxicity of Cd to PSII. The decrease of non-photochemical quenching coefficient (NPQ) indicated the increase of photochemical reaction efficiency with more absorbed light energy for photochemical reactions. Fourier Transform Infrared (FTIR) spectra showed that the foliar treatment stimulated the syntheses of lignin, lipids, aliphatic acid, polysaccharides, carboxylate and proteins. Micrographs of transmission electron microscope (TEM) also revealed the reduced mobility of Cd in cells. Foliar application of S effectively reduced the damage of Cd stress by maintaining the integrity of cell structure and participating in metabolic activities such as protein synthesis.