Protective Effect of Ethanolic Extract of Saussurea Lappa on Paracetamol Induced Toxicity in Female Rabbits

Abstract

The current study was carried out to investigate the protective effects of ethanolic extract of Saussurea lappa on paracetamol induced toxicity in female rabbits. Eighteen female rabbit adult were divided into three groups each (group/6 rabbits). First group (negative control) was administered distilled water for 14 day. Second group (positive control) was administered paracetamol at dose 300 mg/kg B.W orally for 14 day. Third group was administered paracetamol at dose 300 mg/kg B.W orally and treated with ethanolic extract of Saussurea lappa at dose 300 mg/kg B.W orally for 14 day. The animals were sacrificed and blood samples were collected from heart by cardiac puncture. These samples were used for the measurement of biochemical parameter like ALT, AST, TP and Albumin as well as hormonal assay in addition to histological examination for liver, ovary and uterus. The obtained results showed a significant increase (P⩽ 0.05) in ALT, AST and significant decrease (P⩽ 0.05) in TP, Albumin, FSH and LH level in control positive group compared with control negative group while the results showed a significant decrease (P⩽ 0.05) in ALT, AST and increase TP, Albumin, FSH and LH level in rabbits treated with ethanolic extract of Saussurea lappa. Histological studies showed many pathological changes in liver, ovary and uterus in control positive group but in the Saussurea lappa group the histopathological changes near to the normal status. The protective effects of Saussurea lappa extract on paracetamol induce toxicity resulting in improvement effect on liver and reproductive system could be attributed to their antioxidant actions.