Abstract
BackgroundThe protective effect of Erythropoietin (EPO) analogue rHuEPO on acute renal injury induced by exhaustive exercise had been reported. The purpose of this study is to probe into the protective effect of EPO on chronic renal injury induced by repeated exhaustive exercise for four weeks.MethodsEighty adult male Sprague–Dawley rats were used in this experiment. The animals were randomly allocated to one of four groups: control (C), exhaustive exercise test (ET), ET plus EPO pre-treatement (ET+EPO) and ET+EPO plus LY294002 pretreatment (ET+EPO+LY).ResultsCompared with the rats in control group, there was considerable damage in kidney cells in rats of ET group as revealed by histological and ultrastructural examinations. However, treatment with EPO during the training, the exhaustive running distance was significant increased (P < 0.01), and the pathological changes of kidney cell were much less compared with those of rats without EPO intervention. When LY294002, a specific inhibitor of phospholipids phthalocyanine inositol 3-kinase, was added to the EPO treated rats, the injury changes of renal cell were becoming more pronounced.ConclusionsThe protective effect of EPO on chronic renal injury induced by repeated exhaustive exercise was demonstrated in the present study. We proposed that the effect could be due to inhibiting the cell apoptosis and blocking the formation of interstitial fibrosis via activation of the PI3K/Akt pathway, thus plays role in the endogenous protection of the kidney injury.
Highlights
The protective effect of Erythropoietin (EPO) analogue rHuEPO on acute renal injury induced by exhaustive exercise had been reported
At the end of each exhaustive exercise as group exercise test (ET), the rats appeared listless with gloomy eyes
The findings suggested a severe chronic renal injury were induced by four-week exhaustive exercise and such a pathological process was counteracted by the EPO intervention
Summary
The protective effect of Erythropoietin (EPO) analogue rHuEPO on acute renal injury induced by exhaustive exercise had been reported. The purpose of this study is to probe into the protective effect of EPO on chronic renal injury induced by repeated exhaustive exercise for four weeks. High-intensity work, military training and competitive sports will inevitably cause overload or hypoxia. Kidney is one of the vulnerable organs suffered from the highintensity exercise or overload training, manifesting as cystorrhexis, hematuria, proteinuria, acute renal failure etc. The present study was designed to determine the histological and ultrastructural changes in kidney cell of the rats after relatively long exhaustive exercise, evaluate the preventive and therapeutic effects of EPO on such a pathological conditions to provide empirical evidence for the application of EPO clinically
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