Protective effect of Echinochrome against intrahepatic cholestasis induced by alpha-naphthylisothiocyanate in rats.

Sohair R Fahmy,Amel M Soliman,Wafaa E Abd-El Aal,Nesreen Y Almortada,Dawlat A Sayed

doi:10.1590/1519-6984.192697

Abstract

The present study was designed to evaluate the protective effects of echinochrome (Ech) on intrahepatic cholestasis in rats induced by a single (i.p.) injection of alpha-naphthylisothiocyanate (ANIT) (75 mg/kg body weight). The rats were pre-treated orally for 48hr (one dose / 24hr) with Ech (1, 5 and 10 mg/kg body weight) or ursodeoxycholic acid (UDCA) 80 mg/kg body weight drug then, injected with ANIT. ANIT markedly increased serum activities of alanine amino transaminase (ALT), aspartate amino transaminase (AST) and alkaline phosphatase (ALP), which was accompanied by a massive inflammation of epithelial cells on bile duct at 24h after ANIT injection. ANIT also increased the levels of total protein (TP), total bilirubin (TB), direct bilirubin (DB), indirect bilirubin (IB), however decrease albumin content (ALB). In addition ANIT increased hepatic MDA and NO level and decreased GSH level and GST activity. The Ech exerted hepatoprotective and anticholestatic effects as assessed by a significant decrease in the activities of serum AST, ALT and ALP, and the levels of TP, TB, DB and IB as well as liver MDA level and NO level. In conclusion, Ech was found to possess hepatoprotective effect against intrahepatic cholestasis induced by hepatotoxin such as ANIT.

Highlights

The liver is the most important metabolic organ that plays a major role in the metabolism, detoxification, storage and secretory functions in the body (Swarnalatha and Reddy, 2012)
Cholestasis is a reduction in bile flow that leads to the intrahepatic accumulation of bile acids and other toxic compounds with progression of liver pathology, including hepatocellular injury and fibrosis
Results recorded in table 2 showed that serum alkaline phosphatase (ALP) activity is significantly (P

Summary

Introduction

The liver is the most important metabolic organ that plays a major role in the metabolism, detoxification, storage and secretory functions in the body (Swarnalatha and Reddy, 2012). The liver damage causes biliary fibrosis and cirrhosis and the end-stage of liver. 102 102/111 disease requiring liver transplantation (El-Sisi et al, 2013). Cholestasis is a reduction in bile flow that leads to the intrahepatic accumulation of bile acids and other toxic compounds with progression of liver pathology, including hepatocellular injury and fibrosis Protective effect of Echinochrome against intrahepatic cholestasis

Methods

Results

Conclusion