Protective effect of cycloart-23-ene-3 β, 25-diol (B2) isolated from Pongamia pinnata L. Pierre on vital organs in streptozotocin-nicotinamide induced diabetic mice

Abstract

Objective To evaluate the protective effect of cycloart-23-ene-3β, 25-diol (B2) on vital organs in streptozotocin-nicotinamide induced diabetic mice. Methods Diabetes was induced in mice by streptozotocin (200 mg/kg, i.p.) injected 15 min after nicotinamide (110 mg/kg, i.p.). The mice were divided into following groups; group 1- non-diabetic, group 2- diabetic control and group 3- B2 (1 mg/kg, p.o.). At the end of 28th day, mice were sacrificed by cervical dislocation method. Liver, kidney, heart, eye and brain tissue were isolated. The isolated tissues were trimmed into small pieces and preserved in 10 % formalin for 24 h. Specimens were cut in section of 3-5 μ m in thickness and stained by hematoxyline-eosin stain. Results Microscopic examination of vital organs showed severe alteration in diabetic control group in contrast to absence of any changes or minimum changes in B2 treatment. Conclusions It is concluded that B2 exhibits protection of vital organs by minimizing toxic effects and related abnormalities of diabetic conditions in streptozotocin- nicotinamide induced diabetic mice.