Protective Effect of Commercial Green Tea on Ibuprofen-Induced Glomerular Atrophy in Kidney of Adult Rat.

Abstract

To determine the effect of commercial green tea on ibuprofen-induced glomerular atrophy in kidney of adult rat by using transvertical glomerular diameter as the determining parameter. Laboratory-based experimental study. Department of Anatomy, Army Medical College Rawalpindi in collaboration with National Institute of Health (NIH) Islamabad from December 2016 to January 2017. Thirty Sprague-dawley rats, males and females, were selected and divided into three groups. Each group comprised of 10 animals. Group A was used as control. Group B was given ibuprofen 120 mg/kg bodyweight/day. Group C received extract of Lipton green tea 1 ml/100 mg bodyweight/day in addition to ibuprofen 120 mg/kg. The dose was administered once daily for a period of 9 weeks. Animals were sacrificed 24 hours after administration of last dose. Kidney tissue was processed and stained with H&E. Routine histological study was performed. Change in transvertical glomerular diameter was taken as the defining parameter for determining glomerular atrophy. SPSS version 22 was the tool used for statistical analysis. P-value <0.05 was considered significant. Transvertical glomerular diameter (TVGD) was significantly decreased in both experimental groups B (76.15 +1.85 µm) and C (89.59 +1.87 µm) in comparison with control group A (94.27 +1.82 µm). However, the difference in TVGD between both experimental groups was also significant, asserting the ameliorative effect of green tea against glomerular damage. Ibuprofen caused glomerular atrophy but administration of green tea along with ibuprofen protected against significant reduction in glomerular diameter, thus preventing atrophy.