Abstract
The high exposure to acrylamide (AA) due to smoking and increased consumption of processed and fast foods in recent years, has become one of the health threatening problems. This study examined the effect of lemon juice on inflammation and adipokines in acrylamide-induced oxidative stress in rats. Forty animals were divided into five groups. Toxicity was induced by AA (35mg/kg) for two weeks in all groups except normal control group. After that, lemon juice in three doses was administrated to treatment groups for 4 weeks. Serum levels of adipokines and inflammatory parameters and both serum and liver levels of oxidative stress parameters were measured. The results showed groups were received AA had significant higher levels of malondialdehyde, tumor necrosis factor alpha, leptin and C-reactive protein and lower levels of total antioxidant capacity compared to the negative control group. Lemon juice in all three doses significantly improved serum levels of TAC, MDA, TNFα and hs-CRP in treated groups. Also, 7.5 ml/kg lemon juice significantly decreased leptin levels. However, lemon juice had no significant effect on adiponectin levels. This study suggests lemon juice as a potential dietary alternative could attenuate leptin levels and manage oxidative and inflammatory damages in acrylamide- induced toxicity in rats.
Highlights
Acrylamide (AA) or 2-propenamide is an unsaturated and reactive compound (Schwend et al, 2009)
After an acclimatization period of 2 weeks, the experimental animals were randomly divided into five groups (n=8) and treated as follows: I) Negative control received distilled water II) Positive control received AA III) AA- intoxicated rats treated with 2.5 ml/kg lemon juice IV) AA- intoxicated rats treated with 5.0 ml/kg lemon juice V) AA- intoxicated rats treated with 7.5 ml/kg lemon juice Oxidative stress in rats was induced orally by gavage
Effect of citrus lemon administration on serum and liver oxidative stress The results showed that the AA administration decreased both serum and liver total antioxidant capacity (TAC) levels significantly in the positive control group compared to the negative control group (P < 0.01 and P < 0.01, respectively)
Summary
Acrylamide (AA) or 2-propenamide is an unsaturated and reactive compound (Schwend et al, 2009). According to the International Agency for Research on Cancer (IARC), AA is a probable human carcinogen factor (Besaratinia, Pfeifer, 2007) It is not found naturally in food sources. The antioxidant activity of eriocitrin is more potent than other citrus flavonoids (Miyake et al, 2007), so we conducted the current study on the lemon as a rich source of this antioxidant. Antioxidant and anti-inflammatory effects of citrus flavanone, eriocitrin, and hesperidin began to receive attention among researchers. To the best of our knowledge, no previous study has reported the effects of citrus lemon on inflammatory biomarkers and adipocytokines in AA exposure. This study was designed to investigate the effect of lemon juice on inflammation and adipokine levels in acrylamide-induced oxidative stress in rats
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
