Protective effect of Centella asiatica against D-galactose and aluminium chloride induced rats: Behavioural and ultra-structural approaches

Abstract

Event Abstract Back to Event Protective effect of Centella asiatica against D-galactose and aluminium chloride induced rats: Behavioural and ultra-structural approaches Musa S. Chiroma1, 2, Mohamad A. Mohd Moklas2*, Mohamad T. Hidayat Baharuldin2, Che Norma M. Taib2, Zulkhairi Amom3 and Saravanan Jagadeesan4 1 University of Maiduguri, Nigeria 2 Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia 3 Universiti Teknologi MARA Puncak Alam, Malaysia 4 Universiti Tunku Abdul Rahman, Malaysia Background Alzheimer’s disease (AD) is a neurodegenerative disorder and the commonest cause of dementia among the aged people. D-galactose (D-gal) is a senescence agent while aluminium is a known neurotoxin which is linked to pathogenesis of AD. The combined treatments of rats with D-gal and aluminium chloride (AlCl3) is considered as an easy and a cheap method to obtain an animal model of AD. The plant Centella asiatica (CA) is reported to exert neuroprotective effects both in vitro and in vivo. Therefore, this study explores the protective effect of CA on cognition and brain ultra-structure in D-gal and AlCl3 induced rats. Methods Rats were exposed to D-gal 60mg/kg/bw/day + AlCl3 200mg/kg/bw/day and Centella asiatica (200, 400 and 800mg/kg/bw/day) or with donepezil (approved AD drug) 1mg/kg/bw/day for 70 days. Different cognitive paradigms; T maze spontaneous alternation, modified elevated plus maze, and novel object recognition task were used to evaluate full lesion of the hippocampus, spatial learning and memory and non-spatial learning and memory respectively. Nissl’s staining was used to determine the survival of hippocampus CA1 pyramidal cells while transmission electron microscopy was used to check the ultra-structural changes. Results Our results showed that D-gal and AlCl3 could significantly impair behaviour and cognitive functions and cause damage to the hippocampal CA1 pyramidal neurons in rats. In addition, it also caused ultra-structural morphological alterations in the rat’s hippocampus. Conversely, co-administration with CA irrespective of dosage used alleviates the cognitive impairments and the pathological changes in the rats which is comparable to donepezil. Conclusion Taken together, these results suggest that CA could protect against cognitive impairments and morphological alterations caused by D-gal and AlCl3 toxicity in rats. Biochemical and molecular studies are ongoing to elucidate the probable pharmacodynamics of CA. Acknowledgements The authors would like to acknowledge Universiti Putra Malaysia for funding this research project (Grant number GP-IPS 9535400). Keywords: Centella asiatica, cognitive impaiment, Hippocampus, Alheimer's disease, Morphological alteration Conference: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019. Presentation Type: Oral Presentation Topic: Neurodegenerative diseases Citation: Chiroma MS, Mohd Moklas MA, Hidayat Baharuldin MT, Taib CM, Amom Z and Jagadeesan S (2019). Protective effect of Centella asiatica against D-galactose and aluminium chloride induced rats: Behavioural and ultra-structural approaches. Front. Pharmacol. Conference Abstract: International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18) “Seizing Opportunities and Addressing Challenges of Precision Medicine”. doi: 10.3389/conf.fphar.2018.63.00065 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Oct 2018; Published Online: 17 Jan 2019. * Correspondence: Dr. Mohamad A Mohd Moklas, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia, aris@upm.edu.my Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Musa S Chiroma Mohamad A Mohd Moklas Mohamad T Hidayat Baharuldin Che Norma M Taib Zulkhairi Amom Saravanan Jagadeesan Google Musa S Chiroma Mohamad A Mohd Moklas Mohamad T Hidayat Baharuldin Che Norma M Taib Zulkhairi Amom Saravanan Jagadeesan Google Scholar Musa S Chiroma Mohamad A Mohd Moklas Mohamad T Hidayat Baharuldin Che Norma M Taib Zulkhairi Amom Saravanan Jagadeesan PubMed Musa S Chiroma Mohamad A Mohd Moklas Mohamad T Hidayat Baharuldin Che Norma M Taib Zulkhairi Amom Saravanan Jagadeesan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.