Protective effect of arabinose and sugar beet pulp against high glucose-induced oxidative stress in LLC-PK1 cells

Abstract

The protective effects of L-arabinose and sugar beet (SBP) on high glucose-induced oxidative stress were investigated using LLC-PK cells. Under the high glucose-induced cellular oxdative model, the treatment of 45 mM of glucose led to the decrease in cell viability and increase in lipod peroxidation_However. L-arabinose and SBP significantly inhibited the high glucose-induced cytotoxicity and lipid per-oxidation. In addition, the formation of nitric oxide(NO) was increased by the treatment of 45mM glu-cose. while the treatment of L-arabinose or SBP inhibited signigicantly the NO formation compared with high glucose-treated control. The superoxide anion production of groups treated whth L-arabinose or SBP was significantly lower than that of the control treated with high glucose. Futhermore. L-arabinose and SBP elevated the glucose uptake. resulting in lower glucose concentration compared with non-treated control. High glucose levels induced the overexpressions of bax. inducible NO synthase and cyclooxygen-ase-2. but L-arabinose or SBP treatrnent down-regulated the expressions of these genes. Arabinose and SBP also inhibited the expression of nuclear factor-kappa B induced by 45mM glucose in LLC-PK cells. In particular, arabinose exhibited stronger inhibitory activities on high glucose-induced oxdative stress than SBP did. These findings indicate that L-arabinose and SBP are promising antioxidative agents with protective activities against hyperglycemia.