Abstract
Objective To observe the protective effect of aprotinin on astrocytes damaged by thrombin. Methods Cultured astrocytes of rats were divided into five groups randomly，normal control group， thrombin group and aprotinin pretreatment group. After twenty-four hours， the activity of lactate dehydrogenase was determined by chemistry reaction in the culture system. The viability of astrocytes (OD value) was measured by methylthiazolyl tetrazolium (MTT). The apoptotic cells in different aprotinin concentrations were detecte by TUNEL.Results the OD values in the intervention groups of aprotinin (0.67±0.11， 0.78±0.07 and 0.80±0.13) were higher than the thrombin group (1.05±0.09) (P<0.05)； The LDH activities in the intervention groups of aprotinin (154.31±10.74， 101.38±13.59 and 92.72±11.92) were significantly lower than the thrombin group (267.09±25.35) (P<0.05).The apoptotic cells in the intervention groups of aprotinin (13.20±1.52， 9.10±1.20 and 4.80±0.89) was significantly lower than the thrombin group (26.10±1.82) (P<0.05).Conclusions Aprotinin can alleviate the astrocytes damage induced by thrombin by increasing viability and decrease apoptosis of astrocytes， to achieve its protective effect.
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