Protective Effect of Angelica sinensis Extract and Origanum majorana Oil on Hepatic and Renal Toxicities Induced by Nickel Chloride in Male Albino Rats

Abstract

The present study was accomplished to evaluate the efficacy of Angelica sinensis root extract and Origanum majorana oil on subchronic toxicity of nickel chloride (NiCl2) in albino rats by studying the changes in the biochemical parameters, lipid peroxidation and antioxidant status related to the liver and kidneys. This study was carried out on sixty male albino rats with average body weight (120 ± 10 g), which were divided into 6 equal groups. Gp.(1): were kept as control on distilled water, rats in Gp. (2) received NiCl2 (0.75 mg/kg BW), Gp. (3) received A. sinensis root extract (300 mg/kg BW) and Gp. (4) received O. majorana oil (0.5 ml/kg BW). NiCl2 intoxicated rats in Gp. (5) and Gp. (6) were administered A. sinensis root extract and O. majorana oil at the same former doses, respectively. The treatments were daily and orally for 4 weeks. Blood and tissue samples were collected for performing the different experimental analysis after 4 weeks from starting the treatments. Comparing with the control group, results showed that NiCl2 significantly increased serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activities and serum bilirubin, triglycerides, total cholesterol, urea and creatinine concentrations, besides hepatic and renal malondialdehyde concentration, while serum total proteins, albumin and hepatic and renal glutathione, glutathione peroxidase, superoxide dismutase and catalase levels were significantly decreased. A. sinensis root extract and O. majorana oil has no adverse effects and worked on amelioration the various alterations in estimated analytes in combined treated groups. In conclusion, NiCl2 caused hepatic and renal injuries besides the induction of lipid peroxidation and reducing the antioxidant enzymes activities in these organs. Moreover, both of A. sinensis root extract and O. majorana oil provided significant protection against harmful effects of NiCl2 with superiorty of A. sinensis root extract