Abstract
Oxidative stress is one of the key phenomena behind the most common types of chronic diseases. Therefore, the modulation of oxidative stress is an interesting target for acting either through prevention or as a therapeutic approach. In this work, a Portuguese variety of cherry (Saco Cherry) was processed in order to obtain a potent in vitro antioxidant phenolic-rich extract (Ch-PRE), which was further explored to evaluate its potential application as nutraceutical agent against cellular oxidative stress damage. Ch-PRE was mainly composed of anthocyanins, particularly cyanidin-3-rutinoside, cyanidin-3-glucoside, peonidin-3-glucoside and neochlorogenic acid, and exhibited a potent chemical antioxidant activity expressed by its oxygen radical absorbance capacity (ORAC) and hydroxyl radical averting capacity (HORAC) values. Ch-PRE also displayed effective intracellular radical scavenging properties in intestinal epithelial and neuronal cells challenged with oxidative stress but showed a different order of effectiveness regarding the modulation of endogenous antioxidant system. Ch-PRE could be an attractive candidate to formulate an agent for the prevention of oxidative stress-induced disorders such as intestinal inflammation disorders or with an appropriated delivery system for neurodegenerative diseases.
Highlights
Oxidative stress is characterized by an unbalance in reactive oxygen species (ROS) production and the antioxidant defense system that leads to an intracellular accumulation of these harmful radicals [1,2,3]
A phenolic-rich fraction (Ch-PRE) was prepared, from non-marketed Saco cultivar cherries aiming at developing added-value ingredients against oxidative stress
Thephenolic fraction of hydro-alcoholic extract was adsorbed on the polymeric resin and the majority of other constituents such as carbohydrates, organic acids and minerals were discharged on supernatant
Summary
Oxidative stress is characterized by an unbalance in reactive oxygen species (ROS) production and the antioxidant defense system that leads to an intracellular accumulation of these harmful radicals [1,2,3]. Important cellular biomolecules such as proteins, lipids and DNA are damaged by ROS leading to a deficient or complete loss of function of these molecules with deleterious consequences [2]. In this context, oxidative stress is pointed as an important and common mechanism underlying most known types of chronic and degenerative diseases such as diabetes, cardiovascular diseases, chronic intestinal inflammation, cancer and neurodegenerative disorders. Recent studies show that patients with chronic intestinal inflammation have a low activity of endogenous antioxidant systems, which reinforce the importance of oxidative stress in these diseases [5,7]
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