Abstract
A fucose-rich fucoidan was purified from brown seaweed Saccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular reactive oxygen species levels and the viability of UVB-irradiated HaCaT cells were determined. The results indicate that the purified fucoidan significantly reduced the intracellular reactive oxygen species levels and improved the viability of UVB-irradiated HaCaT cells. Furthermore, the purified fucoidan remarkably decreased the apoptosis by regulating the expressions of Bax/Bcl-xL and cleaved caspase-3 in UVB-irradiated HaCaT cells in a dose-dependent manner. In addition, the in vivo UV protective effect of the purified fucoidan was investigated using a zebrafish model. It significantly reduced the intracellular reactive oxygen species level, the cell death, the NO production, and the lipid peroxidation in UVB-irradiated zebrafish in a dose-dependent manner. These results suggest that purified fucoidan has a great potential to be developed as a natural anti-UVB agent applied in the cosmetic industry.
Highlights
Human skin is one of the most fundamental organs which is directly exposed to the external environment
UV is beneficial to the human body to a certain extent, the excessive exposure to UV will damage the ability of basal keratinocytes, which are responsible for maintaining skin homeostasis to resist UV-induced damage, which can lead to different skin diseases such as accelerated degradation of collagen, inflammatory reaction, epidermal hyperplasia and skin cancers [2,3]
Fucoidan fractions of LJSF1-LJSF4 were purified from S. japonica (Figure 1A), among which LJSF4 has the highest sulfate content of 30.72%
Summary
Human skin is one of the most fundamental organs which is directly exposed to the external environment. It acts as a barrier to prevent the body from external damage, including ultraviolet (UV) irradiation exposure [1]. UV is beneficial to the human body to a certain extent, the excessive exposure to UV will damage the ability of basal keratinocytes, which are responsible for maintaining skin homeostasis to resist UV-induced damage, which can lead to different skin diseases such as accelerated degradation of collagen, inflammatory reaction, epidermal hyperplasia and skin cancers [2,3]. It is commonly known that the exposure to solar UVB is the major factor causing keratinocyte damage, causing DNA mutations, which can induce photo-aging and skin cancer [5].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
