Protective and Therapeutic Potential of a Novel Phytobiological Formulation of Nutrients in the Treatment of Indomethacin-Induced Gastric Ulcer

Abstract

Gastric ulcers have been considered as a major problem worldwide. This study investigated the protective synergistic effect of a novel nutrient formulation for the protection and treatment of indomethacin-induced gastric ulcer in rat model. 24, 8 w old male rats with a body weight between 220 and 240 g, were divided into four groups and used for this study. Group 1 consists of healthy controls and group 2 consists of ulcerative animals, group 3 consists of ulcerative+phytobiological formulation treatment (80 mg/kg) and group 4 consists of omeprazole (40 mg/kg). Pre-treatment with 80 mg/kg of phytobiological formulation improved the activities of the measured antioxidant enzymes, decreased lipid peroxidation and inflammation, as evidenced by improved surface and glandular epithelium (white arrows) of phytobiological treated group stained with hematoxylin and eosin and tumor necrosis factor alpha immuno-expression when compared with the ulcer group. In conclusion, the mechanism by which this phytobiological formulation prevents indomethacininduced gastric ulcer may be mediated via improving the homeostasis of enzymatic (superoxide dismutase) and nonenzymatic (glutathione) antioxidants, inhibiting (malondialdehyde) and decreasing the formation of inflammatory cytokines (tumor necrosis factor alpha, C-reactive protein content and interleukin-10). Consequently, this phytobiological formulation may be a beneficial nutrient formulation therapy for patients diagnosed with gastric ulceration.