Abstract
The protective activity of melanin-like pigment (MLP) derived from tea was studied using benzidine (BZ) as a DNA-reactive chemical. A wild strain of Drosophila melanogaster, Canton-S, was used to evaluate the toxic effect of BZ and protective power of MLP. Addition of MLP to feed at concentrations of 1.5–15 μg/ml decreased the toxic effect of BZ and increased the survival of the flies of both sexes. By monitoring chemiluminescence intensity, it was found that MLP prevented the production of free radicals due to metabolic transformation of BZ. MLP also prevented the formation of DNA–DNA cross-links and 8-hydroxy-deoxyguanosine (8-OH-dG) DNA adducts. BZ can form free radial intermediates that bind to DNA sites, blocking the expression of genes responsible for the flies’ resistance to harmful factors. MLP exhibited antioxidant activity by binding to these intermediates.
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