Abstract
Abstract: The objective in this work was to investigate the effect of nitric oxide (NO) like protective agent in sesame seeds submitted to different osmotic potentials. The treatments, in total of eight, were water (control), water plus sodium nitroprusside (SNP) and the other treatments with PEG 6000 and PEG 6000 plus SNP: - 0.1 MPa, -0.1MPa +200 µM of SNP, 0.2 MPa, -0.2 MPa +200 µM of SNP, -0.3 MPa and -0.3 MPa, +200 µM of SNP. Were done the following determinations: germination, first count of germination, speed germination index, hypocotyl length, radicle length, dry mass of hypocotyl and radicle. It was quantified the activity of the antioxidative enzymes, superoxide dismutase, catalase, ascorbate peroxidase and total peroxidase. The experimental design was completely randomized with five replications. The water restriction reduced the germination of sesame seeds, however, the presence of nitric oxide (NO) due to the application of SNP, was beneficial, promoting increase in germination, vigor and seedlings. There was an increase of antioxidative enzymes activity in the period of 0 to 24 hours, demonstrating organization of antioxidative system in all long the time. The association of PEG 6000 to SNP, increased the activity of antioxidative enzymes, evidencing an efficient system of elimination of ROS formed during the exposition to water deficit.
Highlights
In the germination process, the first step in the events sequence that culminate in the resumption of embryo growth is the imbibition
One of the consequences of this exhibition is the generation of reactive oxygen species (ROS), which leads to the oxidative stress, what means, there is an increase in the lipid peroxidation and a decrease in the activity of oxygen reactive species (ROS) sequestrant enzymes (Yao et al, 2012), affecting the structures and cell metabolism (Wang et al, 2009)
The sesame seeds, in five replicates of 50, were put to germinate on a paper towel moistened with 3 mL of the solution, referent to the following treatments in Petri dishes: water, water plus sodium nitroprusside (SNP) and the other treatments with PEG 6000 and PEG 6000 plus SNP; - 0.1 MPa, -0.1 MPa +200 μM of SNP, 0.2 MPa, -0.2 MPa +200 μM of SNP, -0.3 MPa and -0.3 MPa, +200 μM of SNP, summing up to a total of eight treatments
Summary
The first step in the events sequence that culminate in the resumption of embryo growth is the imbibition. The seeds imbibition plays a key role in the germination process (Bewley et al, 2013). The water deficit occurrence in plants leads to the reduction of germination speed and the delaying of the seedlings development. Stronger restrictions come to harm the final germination percentage due to the delay or the non-occurrence of the metabolic processes necessary for germination (Rahimi, 2013). Prolonged exposure to water deficit generates several natural reactions in seeds. One of the consequences of this exhibition is the generation of reactive oxygen species (ROS), which leads to the oxidative stress, what means, there is an increase in the lipid peroxidation and a decrease in the activity of oxygen reactive species (ROS) sequestrant enzymes (Yao et al, 2012), affecting the structures and cell metabolism (Wang et al, 2009)
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