Protection evaluation following vaccination with a Mycobacterium abscessus virulence factor in a pulmonary infection cftr-/- mice model

Abstract

Event Abstract Back to Event Protection evaluation following vaccination with a Mycobacterium abscessus virulence factor in a pulmonary infection cftr-/- mice model Vincent LE MOIGNE1*, Céline GOULARD1, Benoît BARTEAU2, Martin Rottman1, Anne-Laure ROUX1, Bruno PITARD2, Jean-Louis HERRMANN1 and Jean-Louis GAILLARD1 1 Université de Versailles, France 2 INSERM/IncellArt, France Cystic fibrosis (CF) is a disease caused by a mutation in the CFTR gene encoding a transmembrane regulator functioning as a Cl channel within the epithelial membrane. The main mutation is linked to the loss by deletion of a Phe in position 508 (ΔF508). Among the CF pathogens, Mycobacterium abscessus (Mabs), a rapid-growing mycobacterium (RGM), is responsible for severe respiratory infections in CF patients. The specific objective of our project was to evaluate the protection induced by a Mabs virulence factor, the phospholipase C (PLC) (MAB_0555), administered under the form of a nanovectorized recombinant DNA in a model of systemic infection (intravenously) and a model of pulmonary (aerosol route) infection using as mouse model the ΔF508 CFTR mice ("CF" mice) and their wild-type counterpart (FVB genetic background Protection was evaluated by CFU counts in the spleen, liver and lungs of animals at day 1, 7, 14 and 21 for the aerosol challenge and day 1, 14 and 45 for the IV challenge. We only observed a protective effect after the aerosol challenge. After the aerosol challenge of FVB wild-type mice, no protective provided by immunization with the nanovectorized PLC gene effect was observed. However, a protective effect was observed in homozygous ΔF508 CFTR mice. A difference was observed at D7, which was confirmed at D21 (p = 0.038). In conclusion, the use of a nanovectorized gene coding for a virulence factor is able to provide local protection and accelerated clearance of M. abscessus in the lungs of “CF” mice. Keywords: mycobacterium abscessus, Cystic Fibrosis, phospholipase C, Immunization, Genetic vaccination Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: LE MOIGNE V, GOULARD C, BARTEAU B, Rottman M, ROUX A, PITARD B, HERRMANN J and GAILLARD J (2013). Protection evaluation following vaccination with a Mycobacterium abscessus virulence factor in a pulmonary infection cftr-/- mice model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00673 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Vincent LE MOIGNE, Université de Versailles, St-Quentin-en-Yvelines, France, vincentlemoigne@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Vincent LE MOIGNE Céline GOULARD Benoît BARTEAU Martin Rottman Anne-Laure ROUX Bruno PITARD Jean-Louis HERRMANN Jean-Louis GAILLARD Google Vincent LE MOIGNE Céline GOULARD Benoît BARTEAU Martin Rottman Anne-Laure ROUX Bruno PITARD Jean-Louis HERRMANN Jean-Louis GAILLARD Google Scholar Vincent LE MOIGNE Céline GOULARD Benoît BARTEAU Martin Rottman Anne-Laure ROUX Bruno PITARD Jean-Louis HERRMANN Jean-Louis GAILLARD PubMed Vincent LE MOIGNE Céline GOULARD Benoît BARTEAU Martin Rottman Anne-Laure ROUX Bruno PITARD Jean-Louis HERRMANN Jean-Louis GAILLARD Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.