Protection by vitamin E, selenium, trolox C, ascorbic acid palmitate, acetylcysteine, coenzyme Q, beta-carotene, canthaxanthin, and (+)-catechin against oxidative damage to liver slices measured by oxidized heme proteins

Abstract

Male SD rats were fed a vitamin E- and selenium-deficient diet, a diet supplemented with vitamin E and selenium, and diets supplemented with vitamin E, selenium, trolox C, ascorbic acid palmitate, acetylcysteine, beta-carotene, canthaxanthin, coenzyme Q 0, coenzyme Q 1, and (+)-catechin. Liver slices were incubated at 37°C with and without CBrCl 3, t-butyl-hydroperoxide, Fe +2, or Cu +2. The effect of antioxidant nutrients on the oxidativedamage to rat liver was studies by measurement of the production of oxidized heme proteins (OHP) during the oxidative reactions. Diet supplemented with vitamin E and selenium showed protection against heme protein oxidation compared to the antioxidatt-deficient diet. Furthermore, increasing the diversity and quantity of antioxidants in the diets provided significantly more protection.