Protection by Ethanolic Extract from Ulva lactuca L. against Acute Myocardial Infarction: Antioxidant and Antiapoptotic Activities.

Wahyu Widyaningsih,Zulaela ,Sitarina Widyarini,Suwidjiyo Pramono,Sugiyanto

doi:10.21315/mjms2017.24.6.5

Wahyu Widyaningsih, Zulaela + Show 3 more

Open Access

https://doi.org/10.21315/mjms2017.24.6.5

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Abstract

Reactive oxygen species (ROS) play a major role in myocardial damage during acute myocardial infarction (AMI). This study aimed to determine the antioxidant and antiapoptotic activities of an ethanolic extract from Ulva lactuca L. (EEUL) against AMI. Thirty-six male Wistar rats were divided into six groups: one control group and five treatment groups. Treatment group II was given 85 mg/kg body weight (BW) of isoproterenol (ISO). Group III, IV and V were given ISO and EEUL at 250, 500 and 750 mg/kg BW, respectively. Group VI were given 10 mg/kg BW of ISO and melatonin. EEUL and melatonin were orally administered for 28 days. ISO was injected subcutaneously on day 29 and 30 to chemically induce AMI. On day 31, blood was collected for antioxidant assay and heart tissues were collected for histological examination. The activity of catalase (CAT), an endogenous antioxidant, in the EEUL-treatment groups was significantly increased compared to the ISO-treatment group (P < 0.001). The EEUL-treatment groups showed significantly decreased expression of caspase-3 (P < 0.001) and better myocardial tissue morphology. EEUL possibly protects against AMI because of its antioxidant and antiapoptotic properties.

Highlights

Acute myocardial infarction (AMI) is responsible for considerable human morbidity and mortality worldwide [1,2]
Treatment with ethanolic extract from Ulva lactuca L. (EEUL) at 250, 500 and 750 mg/kg body weight (BW) and 10 mg/kg BW of melatonin reduced the level of MDA to 11.70%, 11.98%, 23.40% and 6.69%, respectively, compared with the ISO group
Administration of EEUL at 250, 500 and 750 mg/kg BW and melatonin at 10 mg/kg BW significantly increased the level of superoxide dismutase (SOD) activity

Summary

Introduction

Acute myocardial infarction (AMI) is responsible for considerable human morbidity and mortality worldwide [1,2]. Previous studies have reported that reactive oxygen species (ROS) are responsible for myocardial damage during AMI [3]. ROS are reported to induce myocardial apoptosis, which plays an important role in the pathogenesis of cardiac diseases, including myocardial infarction [6,7]. Melatonin has been demonstrated to have a cardioprotective effect in male rat SD induced by ISO and antiadrenergics [11]. Endogenous antioxidants, such as SOD, glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase and nitric oxide synthases, are significantly increased by administration of melatonin in rat models [12]. Reactive oxygen species (ROS) play a major role in myocardial damage during acute myocardial infarction (AMI). This study aimed to determine the antioxidant and antiapoptotic activities of an ethanolic extract from Ulva lactuca L. (EEUL) against AMI

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