Abstract

Plasmid DNA vaccines pcDNA-VP2 expressing only VP2 protein and pcDNA-VP243 expressing VP2, VP4 and VP3 proteins of very virulent infectious bursal disease virus (vvIBDV) Korean SH/92 strain were constructed. The expression of viral proteins from constructed DNA vaccines was confirmed by an in vitro transcription/translation system and transfection in COS-7 cells. To investigate the protective efficacy of these DNA vaccines, 2-week-old chickens were injected intramuscularly and intraperitoneally with pcDNA-VP2 and pcDNA-VP243 twice at 2-week intervals. On week 2 after the second immunization, chickens were orally challenged with the vvIBDV SH/92 strain and observed for 10 days. Antibodies specific to IBDV were not detected by enzyme-linked immunosorbent assay in DNA vaccination groups before challenge but were induced after challenge. The immunized groups exhibited a higher survival rate and lower bursal atrophy as compared with the non-immunized groups after challenge. The survival rates of pcDNA-VP243 and pcDNA-VP2 groups were 70 and 50%, respectively, but the survival rate of challenge control group was only 10%. In the ConA-induced lymphocyte proliferation assay of peripheral blood and splenic lymphocytes, the immunized groups showed significantly higher proliferation responses ( P<0.05) than non-immunized groups. The maintenance of T cell proliferation activity in DNA vaccination groups may be closely related to the protection against vvIBDV. These results suggest that our plasmid DNA vaccines induced high protective immunity against vvIBDV, in which cell mediated immune response rather than humoral immune response seemed to contribute to the protection of chickens against vvIBDV infection.

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