Abstract
Studies have implicated oxygen radicals in the etiology of necrotizing enterocolitis (NEC). DM, a membrane stabilizer, may protect against oxidant injury of cells. We assessed the role of DM in gut ischemia and reperfusion, which produces experimental NEC. Laparotomy under anesthesia was performed in 16 rabbits. In each, 4 intestinal loops, 5 cm long, were prepared. Only 2 of these loops were rendered ischemic for 5 min., the other 2 loops serving as non-ischemic controls. 8 rabbits were pre-treated with IV DM (0.1 mg/kg); in the other 8, Intraluminal (IL) DM (1 mg) was injected into 2 loops while the other 2 were injected with saline (S). The animals were sacrificed 4 hrs. after surgery. All ileal loops were fixed and examined histologically by a single pathologist unaware of group assignment. Histological changes were graded. The non-ischemic loops were normal. DM (IV & IL) was fully protective against reperfusion injury, with two exceptions. These dramatic results suggest that DM may prevent experimental intestinal necrosis.
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