Proteases Secreted by the Infective Larvae of Toxocara canis and Partial Purification of a 50 kDa Protease

Abstract

with subclinical leishmaniasis, and 100 seronegative healthy controls were measured by enzyme-linked immunosorbent assay. Genotyping of CD 28 gene polymorphism was performed by polymerase chain reaction based allotyping method using allele-specific primers for C or T at intron 3 position +17 in three groups. Results: The frequency of CC genotype was significantly higher in subclinical VL patients (42.4%) than active VL group (27.3%) and healthy controls (16%) (p < 0.001). Also, the frequency of allele C among subclinical VL group (57.6%) was significantly higher than active VL (40.9%) and control groups (34%) (p = 0.003). No significant differences were observed between the plasma levels of sCD 28 in three groups. Conclusion: Our findings suggest that the CD 28 gene may have significant role in the protection of active VL in the Iranian population.