Proteases and keratinases from Bacillus zhangzhouensis MH1: Practical use in detergent, leather, and waste management processes

Abstract

Microbial proteases and keratinases find extensive application in both the detergent and leather industries, as well as in poultry waste management. In this study, a multifunctional strain MH1 exhibiting proteolytic and keratinolytic activities was newly isolated and identified as Bacillus zhangzhouensis. To improve its stability, the proteolytic extract was spray-dried and the stability was assessed during two years of storage. The enzyme preparation was fully stable up to 20 months of conservation at 4 °C even in the absence of any protective agent, while the enzymatic half-life at room temperature was twenty months using maltodextrin as a protector additive. MH1 was a feather-decomposing strain producing keratinases (95 U/ml) on feather medium. Therefore, the study evaluated the use of these enzymes in the detergent, tannery, and feed processes. Results showed that the sprayed proteases showed high compatibility with commercial liquid and solid detergents and efficiently removed bloodstains at low wash temperatures. They also revealed significant dehairing activity of cow skin without surface damage. While keratinases effectively transformed chicken feathers into keratin hydrolysate with strong antioxidant activity. Therefore, these enzymes could be a green alternative to hazardous chemicals utilized for detergent, leather, and biodegradation of keratinous waste.