Prosthetic Group of Aldehyde Dehydrogenase in Acetic Acid Bacteria Not Pyrroloquinoline Quinone

Abstract

A mutant strain, G7, of Acetobacter sp. BPR2001 incapable of producing pyrroloquinoline quinone (PQQ) was obtained by chemical mutagenesis. The strain was defective in the activities of membrane-bound glucose dehydrogenase and alcohol dehydrogenase, both of which known to contain PQQ as their prosthetic group. However, the mutant had almost the same level of activity as the parental strain of membrane-bound aldehyde dehydrogenase, which also has been suggested to be a quinoprotein with PQQ. Our findings showed that PQQ was not the prosthetic group of membrane-bound aldehyde dehydrogenase.