Prostate Adenocarcinoma: Effects of Castration on in Situ Androgen Uptake by Individual Cell Types

Abstract

The cellular distribution of androgen uptake was investigated in normal prostate and in Dunning R3327H prostate tumors in Copenhagen rats in vivo at different time intervals after castration. Quantitative dry-autoradiography was used to demonstrate which cell types have androgen binding, and to quantify the amount of androgen binding per cell type during initial castration-induced tumor regression and subsequent tumor relapse. Regardless of time after castration, tumor stromal nuclei had significantly more 3H-dihydrotestosterone (DHT) labelling than did tumor epithelial nuclei (p <.001). On the other hand, prostate gland epithelial nuclei showed more 3H-DHT binding than prostate gland stromal nuclei. Tumor stromal nuclei had greater DHT uptake than any other cell type measured in the tumor or in secondary sex organs at all times after castration. Different cell types responded differently to castration. The 3H-DHT uptake measured in tumor stromal nuclei after castration showed that one day after castration 14.6 ± 2.1 silver grains were present, 14 days after castration 9.3 ± 2.4 were seen, and 50 days after castration 18.9 ± 1.8 were present. This significant increase from 14 days to 50 days is not seen in the other cell types. This study gives insight into the cellular androgen receptor distribution in normal and malignant rat prostate tissue.