Abstract
A discrepancy between published values of PGI 2 production by human umbilical artery in vitro measured by platelet bioassay, compared with values of 6-oxo-PGF 1α by radioimmunoassay, raised the possibility that another anti-aggregatory prostanoid was produced by this tissue. To test this hypothesis, umbilical artery rings were incubated in buffer and PGI 2 determined by platelet bioassay and by a more specific radioimmunoassay based on comparison of 6-oxo-PGF 1α in hydrolysed and non-hydrolysed samples. 6-oxo-PGF 1a, PGF 2α and TXB 2 were also measured by gas chromatography negative ion chemical ionisation mass spectrometry. PGI 2 concentrations by radioimmunoassay and bioassay were significantly correlated (r = 0.92, p < 0.01). There was no difference between them, disproving the presence of an additional antiaggregatory substance. PGI 2 production determined by bioassay (mean 1.21 ng/mg wet weight/h, range 0.59–1.53 ng/mg/h) differed from previously reported values (range 70–325 ng/mg/h). 6-oxo-PGF 1α concentrations were confirmed by gas chromatography negative ion chemical ionisation mass spectrometry. Previous determinations of PGI 2 production by this tissue overestimated it by approximately 100 times.
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