Prostaglandins have limited actions on abnormalities of beating induced in cultured heart cells

Abstract

Prostaglandins are antiarrhythmic in a variety of situations including ischaemic arrhythmias, but the mechanisms involved are not known. In view of this, the protective actions of prostaglandins A 2, E 2, F 1α, F 2β, and I 2 against abnormalities of beating induced in cultured heart cells were investigated. Abnormalities of beating were induced in single cells by a variety of agents including ouabain, Ca ++, K +, dinitrophenol (DNP), and toxic material from the jellyfish Cyanea . Abnormalities were assessed in terms of rate, rate range, subjective arrhythmic behaviour and percent cells beating. The prostaglandins (at 10 −7-10 −5 M) were added with the arrhythmogenic agent to test for their ability to modify agent-induced beating abnormalities and were compared with lidocaine and quinidine. Prostaglandins alone had minimal direct effects on the cells and only minimally reduced responses to arrhythmogenic agents. The most protective prostaglandins, PGE 2 and PGF 1α, tended to normalise beating behaviour most noticeably in DNP-treated cells, unlike lidocaine and quinidine which were effective against Ca ++-induced changes while worsening those of K +. Thus, a general ability to protect disturbed cardiac cells is not seen with high concentrations of prostaglandins.