Prostaglandin-induced secretion of oxytocin and prolactin in red ( Cervus elaphus) and Pere David's ( Elaphurus davidianus) deer hinds: Evidence for oxytocin of luteal origin

Abstract

Peripheral plasma concentrations of oxytocin in female red deer during the luteal phase of the oestrous cycle (9.3 ± 2.1 fmol/ml) exceeded those in the follicular phase (3.1 ± 1.4) or during seasonal anoestrus (3.2 ± 1.3). In both red and Père David's deer hinds during the mid-luteal phase of the cycle, systemic administration of a luteolytic dose of the prostaglandin F 2α analogue, cloprostenol, caused the concentration of oxytocin in the peripheral circulation to rise. Mean (±SEM) concentrations increased from 8.1 ± 0.7 to 97 ± 8 fmol/ml in red and from 6.2 ± 0.7 to 153 ± 30 fmol/ml in Père David's hinds within 5 min of treatment. During seasonal anoestrus oxytocin secretion in response to cloprostenol was reduced to less than 10% of that during the breeding season, in both species. Cloprostenol treatment raised circulating concentrations of prolactin in both species during the breeding season, and during anoestrus in red deer only. The concentration of oxytocin in a single corpus luteum removed at laparotomy from one red deer hind at the mid-luteal phase of the cycle was 66 nmol/g wet wt; identification was authenticated by HPLC. These results suggest that the corpus luteum secretes oxytocin in the Cervidae, as established previously in the Bovidae, and that luteal oxytocin secretion is stimulated by prostaglandin.