Abstract
Uteri, ovaries and oviducts from mice were collected at autopsy. Tissue slices were incubated with [ 3H]-PGE 2 in the presence or absence of a large excess (100 fold) of nonradioactive PGE 2 using 0.01M sodium phosphate buffer (pH 7.2). Bound and free PGs were separated by a filtration technique. PGE 2 accumulation by the uteri was evaluated as a function of incubation time, wet weight of tissues, and reproductive state. The tissue to medium ratio (T/M) was greater than 1.0 for uteri as the time of incubation increased. This suggests the presence of PGE 2 binding sites in mouse uterine tissue. Also, PGE 2 accumulation was not observed in oviducts or in ovaries.
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