Abstract
PGE 2 induced shape changes in porcine adventitial fibroblasts grown on glass in low density monolayer cell cultures. Incubation of the cells with PGE 2 at concentrations of 100 ng/ml and 1000 ng/ml induced rounding of flat fibroblasts within one hour. The rounded cells had a small rim of cytoplasm around the nucleus and from one to several long thin arborizing cytoplasmic processes extending outward along the substratum. Removal of the PGE 2 resulted in transient blebbing of the cell membrane of both the cell body and the processes as the cells returned to their flat normal morphology within one hour. The effect could be inhibited by 1% fetal calf serum. PGF 2α did not however induce similar changes. This difference between PGE 2 and PGF 2α is similar to a report on spreading and migration of mouse peritoneal macrophages, and suggests that under certain conditions PGE 2 may have the ability to induce shape changes in cells.
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