Prostaglandin H synthase-2 induction by hydroxy-eicosa-tetraenoic acids (HETES) in an intestinal subepithelial myofibroblast cell line

Abstract

immunohistochemically examined for the expression of enteroglial markers at several different passages. All 5 cell lines expressed robust GFAP, S-100 and vimentin immunoreactivities, but no cell line expressed the mesenchymal markers desmin (smooth muscle cell differentiation marker) or Thy-l.1 (expressed on rodent fibroblasts). To further confirm these findings, dot blot analysis of the cytosolic and membrane protein fraction of each cell line was performed showing expression of GFAP and S-100 in the cytosolic fraction, while no Thy-l.1 expression could be detected. These findings indicate that immortalized enteroglial cells display the same phenotype as cells from primary cultures, suggesting that they are physiologically resembling primary cells. In summary, we are presenting the first immortalized enteroglial cell lines retaining several of the properties that are typical for primary EGC. These cell lines appear to be useful models for detailed investigations of the functional properties of EGC in vitro. Supported by DFG Ru 528/5.